Using Luminescent Nanoparticles as Staining Probes for Affymetrix GeneChips
نویسندگان
چکیده
منابع مشابه
Gene filtering by PCA for Affymetrix GeneChips
Due to the nature of the array experiments which examine the expression of tens of thousands of genes (or probesets) simultaneously, the number of null hypotheses to be tested is large. Hence multiple testing correction is often necessary to control the number of false positives. However, multiple testing correction can lead to low statistical power in detecting genes that are truly differentia...
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The reliable interpretation of Affymetrix GeneChip data is a multi-faceted problem. The interplay between biophysics, bioinformatics and mining of GeneChip surveys is leading to new insights into how best to analyse the data. Many of the molecular processes occurring on the surfaces of GeneChips result from the high surface density of probes. Interactions between neighbouring adjacent probes af...
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We have previously discovered that probes containing runs of four or more contiguous guanines are not reliable for measuring gene expression in the Human HG_U133A Affymetrix GeneChip data. These probes are not correlated with other members of their probe set, but they are correlated with each other. We now extend our analysis to different 3' GeneChip designs of mouse, rat, and human. We find th...
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ژورنال
عنوان ژورنال: Bioconjugate Chemistry
سال: 2007
ISSN: 1043-1802,1520-4812
DOI: 10.1021/bc060365u